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oroboros o2k fluorometer  (Oroboros Instruments)

 
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    Oroboros Instruments oroboros o2k fluorometer
    (A) Mitochondrial respiration and H 2 O 2 production were measured using an Oroboros <t>O2k</t> fluorometer in mitochondria isolated from WT and HuR Hep−/− livers under untreated (control) conditions and at 2 h after APAP treatment (2 h APAP). Oxygen consumption was assessed sequentially following the addition of malate, ADP, succinate, and FCCP. n = 3–4 mice per group. (B) Mitochondrial redox status, expressed as basal H 2 O 2 production normalized to basal O 2 flux, and OXPHOS capacity, calculated as State 3 respiration minus leak respiration. n = 3–4 mice per group. (C) Representative transmission electron micrographs (TEMs) of WT and HuR Hep−/− mouse livers collected under control conditions and at indicated time points following APAP treatment. TEM analysis was performed using one mouse per genotype and time point. Mitochondrial features were quantified at the organelle level and are presented as within-animal distributions. Mitochondrial number represents the total number of mitochondria per micrograph, whereas mitochondrial area represents the total surface area occupied by mitochondria in each micrograph. (D) Western blot analysis of mitochondrial dynamics proteins Opa1, Mfn2, and Drp1 in livers collected 2 h after APAP treatment. Protein levels were normalized to GAPDH. Data are represented as mean ± SEM. n = 5–6 mice per group. Each data point represents an individual mouse. Statistical differences between 2 groups were analyzed using an unpaired 2-tailed Student’s t test. Comparisons involving multiple genotypes and time points were analyzed using two-way ANOVA followed by Sidak’s multiple comparisons test. * p < 0.05, ** p < 0.01. Abbreviations: ADP, adenine diphosphate; FCCP, carbonyl cyanide-p-(trifluoromethyoxy) phenylhydrazone; M, mitochondria; N, nucleus, Opa1, optic atrophy 1; Mfn2, mitofusion 2; Drp1, dynamin-related protein 1.
    Oroboros O2k Fluorometer, supplied by Oroboros Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/o2k+fluorometer/high+resolution+respirometry/pmc13202677-106-13-16
    Average 86 stars, based on 1 article reviews
    oroboros o2k fluorometer - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "Dysregulation of xenobiotic metabolism and mitochondrial dysfunction exacerbate acetaminophen-induced hepatotoxicity in human antigen R-deficient male mice"

    Article Title: Dysregulation of xenobiotic metabolism and mitochondrial dysfunction exacerbate acetaminophen-induced hepatotoxicity in human antigen R-deficient male mice

    Journal: Toxicology

    doi: 10.1016/j.tox.2026.154480

    (A) Mitochondrial respiration and H 2 O 2 production were measured using an Oroboros O2k fluorometer in mitochondria isolated from WT and HuR Hep−/− livers under untreated (control) conditions and at 2 h after APAP treatment (2 h APAP). Oxygen consumption was assessed sequentially following the addition of malate, ADP, succinate, and FCCP. n = 3–4 mice per group. (B) Mitochondrial redox status, expressed as basal H 2 O 2 production normalized to basal O 2 flux, and OXPHOS capacity, calculated as State 3 respiration minus leak respiration. n = 3–4 mice per group. (C) Representative transmission electron micrographs (TEMs) of WT and HuR Hep−/− mouse livers collected under control conditions and at indicated time points following APAP treatment. TEM analysis was performed using one mouse per genotype and time point. Mitochondrial features were quantified at the organelle level and are presented as within-animal distributions. Mitochondrial number represents the total number of mitochondria per micrograph, whereas mitochondrial area represents the total surface area occupied by mitochondria in each micrograph. (D) Western blot analysis of mitochondrial dynamics proteins Opa1, Mfn2, and Drp1 in livers collected 2 h after APAP treatment. Protein levels were normalized to GAPDH. Data are represented as mean ± SEM. n = 5–6 mice per group. Each data point represents an individual mouse. Statistical differences between 2 groups were analyzed using an unpaired 2-tailed Student’s t test. Comparisons involving multiple genotypes and time points were analyzed using two-way ANOVA followed by Sidak’s multiple comparisons test. * p < 0.05, ** p < 0.01. Abbreviations: ADP, adenine diphosphate; FCCP, carbonyl cyanide-p-(trifluoromethyoxy) phenylhydrazone; M, mitochondria; N, nucleus, Opa1, optic atrophy 1; Mfn2, mitofusion 2; Drp1, dynamin-related protein 1.
    Figure Legend Snippet: (A) Mitochondrial respiration and H 2 O 2 production were measured using an Oroboros O2k fluorometer in mitochondria isolated from WT and HuR Hep−/− livers under untreated (control) conditions and at 2 h after APAP treatment (2 h APAP). Oxygen consumption was assessed sequentially following the addition of malate, ADP, succinate, and FCCP. n = 3–4 mice per group. (B) Mitochondrial redox status, expressed as basal H 2 O 2 production normalized to basal O 2 flux, and OXPHOS capacity, calculated as State 3 respiration minus leak respiration. n = 3–4 mice per group. (C) Representative transmission electron micrographs (TEMs) of WT and HuR Hep−/− mouse livers collected under control conditions and at indicated time points following APAP treatment. TEM analysis was performed using one mouse per genotype and time point. Mitochondrial features were quantified at the organelle level and are presented as within-animal distributions. Mitochondrial number represents the total number of mitochondria per micrograph, whereas mitochondrial area represents the total surface area occupied by mitochondria in each micrograph. (D) Western blot analysis of mitochondrial dynamics proteins Opa1, Mfn2, and Drp1 in livers collected 2 h after APAP treatment. Protein levels were normalized to GAPDH. Data are represented as mean ± SEM. n = 5–6 mice per group. Each data point represents an individual mouse. Statistical differences between 2 groups were analyzed using an unpaired 2-tailed Student’s t test. Comparisons involving multiple genotypes and time points were analyzed using two-way ANOVA followed by Sidak’s multiple comparisons test. * p < 0.05, ** p < 0.01. Abbreviations: ADP, adenine diphosphate; FCCP, carbonyl cyanide-p-(trifluoromethyoxy) phenylhydrazone; M, mitochondria; N, nucleus, Opa1, optic atrophy 1; Mfn2, mitofusion 2; Drp1, dynamin-related protein 1.

    Techniques Used: Isolation, Control, Transmission Assay, Western Blot

    Related Articles

    Isolation:

    Article Title: Early Administration of N-Acetylcysteine Provides Renal and Cardiac Mitochondrial and Redox Protection, Preventing the Development of Cardio-Renal Syndrome Type IV Induced by 5/6NX
    Article Snippet: .. Mitochondrial H 2 O 2 rates at 37 °C were evaluated in freshly isolated mitochondria of renal and heart tissues, as previously described [ , ], using O2k-Fluorometer (OROBOROS, Innsbruck, Austria) with Amplex red as a probe. ..



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    (A) Mitochondrial respiration and H 2 O 2 production were measured using an Oroboros <t>O2k</t> fluorometer in mitochondria isolated from WT and HuR Hep−/− livers under untreated (control) conditions and at 2 h after APAP treatment (2 h APAP). Oxygen consumption was assessed sequentially following the addition of malate, ADP, succinate, and FCCP. n = 3–4 mice per group. (B) Mitochondrial redox status, expressed as basal H 2 O 2 production normalized to basal O 2 flux, and OXPHOS capacity, calculated as State 3 respiration minus leak respiration. n = 3–4 mice per group. (C) Representative transmission electron micrographs (TEMs) of WT and HuR Hep−/− mouse livers collected under control conditions and at indicated time points following APAP treatment. TEM analysis was performed using one mouse per genotype and time point. Mitochondrial features were quantified at the organelle level and are presented as within-animal distributions. Mitochondrial number represents the total number of mitochondria per micrograph, whereas mitochondrial area represents the total surface area occupied by mitochondria in each micrograph. (D) Western blot analysis of mitochondrial dynamics proteins Opa1, Mfn2, and Drp1 in livers collected 2 h after APAP treatment. Protein levels were normalized to GAPDH. Data are represented as mean ± SEM. n = 5–6 mice per group. Each data point represents an individual mouse. Statistical differences between 2 groups were analyzed using an unpaired 2-tailed Student’s t test. Comparisons involving multiple genotypes and time points were analyzed using two-way ANOVA followed by Sidak’s multiple comparisons test. * p < 0.05, ** p < 0.01. Abbreviations: ADP, adenine diphosphate; FCCP, carbonyl cyanide-p-(trifluoromethyoxy) phenylhydrazone; M, mitochondria; N, nucleus, Opa1, optic atrophy 1; Mfn2, mitofusion 2; Drp1, dynamin-related protein 1.
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    Image Search Results


    (A) Mitochondrial respiration and H 2 O 2 production were measured using an Oroboros O2k fluorometer in mitochondria isolated from WT and HuR Hep−/− livers under untreated (control) conditions and at 2 h after APAP treatment (2 h APAP). Oxygen consumption was assessed sequentially following the addition of malate, ADP, succinate, and FCCP. n = 3–4 mice per group. (B) Mitochondrial redox status, expressed as basal H 2 O 2 production normalized to basal O 2 flux, and OXPHOS capacity, calculated as State 3 respiration minus leak respiration. n = 3–4 mice per group. (C) Representative transmission electron micrographs (TEMs) of WT and HuR Hep−/− mouse livers collected under control conditions and at indicated time points following APAP treatment. TEM analysis was performed using one mouse per genotype and time point. Mitochondrial features were quantified at the organelle level and are presented as within-animal distributions. Mitochondrial number represents the total number of mitochondria per micrograph, whereas mitochondrial area represents the total surface area occupied by mitochondria in each micrograph. (D) Western blot analysis of mitochondrial dynamics proteins Opa1, Mfn2, and Drp1 in livers collected 2 h after APAP treatment. Protein levels were normalized to GAPDH. Data are represented as mean ± SEM. n = 5–6 mice per group. Each data point represents an individual mouse. Statistical differences between 2 groups were analyzed using an unpaired 2-tailed Student’s t test. Comparisons involving multiple genotypes and time points were analyzed using two-way ANOVA followed by Sidak’s multiple comparisons test. * p < 0.05, ** p < 0.01. Abbreviations: ADP, adenine diphosphate; FCCP, carbonyl cyanide-p-(trifluoromethyoxy) phenylhydrazone; M, mitochondria; N, nucleus, Opa1, optic atrophy 1; Mfn2, mitofusion 2; Drp1, dynamin-related protein 1.

    Journal: Toxicology

    Article Title: Dysregulation of xenobiotic metabolism and mitochondrial dysfunction exacerbate acetaminophen-induced hepatotoxicity in human antigen R-deficient male mice

    doi: 10.1016/j.tox.2026.154480

    Figure Lengend Snippet: (A) Mitochondrial respiration and H 2 O 2 production were measured using an Oroboros O2k fluorometer in mitochondria isolated from WT and HuR Hep−/− livers under untreated (control) conditions and at 2 h after APAP treatment (2 h APAP). Oxygen consumption was assessed sequentially following the addition of malate, ADP, succinate, and FCCP. n = 3–4 mice per group. (B) Mitochondrial redox status, expressed as basal H 2 O 2 production normalized to basal O 2 flux, and OXPHOS capacity, calculated as State 3 respiration minus leak respiration. n = 3–4 mice per group. (C) Representative transmission electron micrographs (TEMs) of WT and HuR Hep−/− mouse livers collected under control conditions and at indicated time points following APAP treatment. TEM analysis was performed using one mouse per genotype and time point. Mitochondrial features were quantified at the organelle level and are presented as within-animal distributions. Mitochondrial number represents the total number of mitochondria per micrograph, whereas mitochondrial area represents the total surface area occupied by mitochondria in each micrograph. (D) Western blot analysis of mitochondrial dynamics proteins Opa1, Mfn2, and Drp1 in livers collected 2 h after APAP treatment. Protein levels were normalized to GAPDH. Data are represented as mean ± SEM. n = 5–6 mice per group. Each data point represents an individual mouse. Statistical differences between 2 groups were analyzed using an unpaired 2-tailed Student’s t test. Comparisons involving multiple genotypes and time points were analyzed using two-way ANOVA followed by Sidak’s multiple comparisons test. * p < 0.05, ** p < 0.01. Abbreviations: ADP, adenine diphosphate; FCCP, carbonyl cyanide-p-(trifluoromethyoxy) phenylhydrazone; M, mitochondria; N, nucleus, Opa1, optic atrophy 1; Mfn2, mitofusion 2; Drp1, dynamin-related protein 1.

    Article Snippet: Mitochondrial respiration and H 2 O 2 emission were measured simultaneously using an Oroboros O2K fluorometer (Oroboros Instruments, Austria) following established protocols ( ; ).

    Techniques: Isolation, Control, Transmission Assay, Western Blot